Hi Prathap
From the existing URL, my guess is the previous setup is using the ABAP HTTP adapter on dual stack. Moving to single stack, you will need to use the Java HTTP_AAE adapter.
The path and query strings are slightly different for the HTTP_AAE adapter, so your RFC destination needs to be changed. Refer to the library below for how the new path should look like.
Configuring the Java HTTP Adapter on the Sender Channel - Advanced Adapter Engine - SAP Library
Rgds
Eng Swee